Co-immunization with IL-15 enhances cellular immune responses induced by a vif-deleted simian immunodeficiency virus proviral DNA vaccine and confers partial protection against vaginal challenge with SIVmac251.
Virology
; 386(1): 109-21, 2009 Mar 30.
Article
en En
| MEDLINE
| ID: mdl-19193388
ABSTRACT
Simian immunodeficiency virus (SIV) infection of rhesus macaques is a valuable animal model for human immunodeficiency virus (HIV)-1 vaccine development. Our laboratory recently described the immunogenicity and limited efficacy of a vif-deleted SIVmac239 proviral DNA (SIV/CMVDelta vif) vaccine. The current report characterizes immunogenicity and efficacy for the SIV/CMVDelta vif proviral DNA vaccine when co-inoculated with an optimized rhesus interleukin (rIL)-15 expression plasmid. Macaques co-inoculated with rIL-15 and SIV/CMVDelta vif proviral plasmids showed significantly improved SIV-specific CD8 T cell immunity characterized by increased IFN-gamma ELISPOT and polyfunctional CD8 T cell responses. Furthermore, these animals demonstrated a sustained suppression of plasma virus loads after multiple low dose vaginal challenges with pathogenic SIVmac251. Importantly, SIV-specific cellular responses were greater in immunized animals compared to unvaccinated controls during the initial 12 weeks after challenge. Taken together, these findings support the use of IL-15 as an adjuvant in prophylactic anti-HIV vaccine strategies.
Texto completo:
1
Banco de datos:
MEDLINE
Asunto principal:
Adyuvantes Inmunológicos
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Síndrome de Inmunodeficiencia Adquirida del Simio
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Provirus
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Vacunas contra el SIDAS
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Linfocitos T CD8-positivos
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Vacunas de ADN
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Interleucina-15
Tipo de estudio:
Prognostic_studies
Límite:
Animals
Idioma:
En
Revista:
Virology
Año:
2009
Tipo del documento:
Article
País de afiliación:
Estados Unidos