[Influence of As(2)O(3) on proteasome beta(1)-subunit in NB4 cells].
Zhongguo Shi Yan Xue Ye Xue Za Zhi
; 17(3): 579-82, 2009 Jun.
Article
en Zh
| MEDLINE
| ID: mdl-19549367
ABSTRACT
This study was aimed to explore the correlation between effects of arsenic trioxide on NB4 cell differentiation and the change of beta(1)-subunit of 26S proteasome. NB4 cell in 0.5 micromol/L As(2)O(3) was incubated for 24 hours and 48 hours, then total protein was extracted, expressions of subunit beta(1) and PML-RARalpha fusion protein were determined by Western blot. The results indicated that the expression of 26S proteasome beta(1)-subunit increased after incubation with As(2)O(3) for 24 hours, but after culture with As(2)O(3) for 48 hours, the expression of beta-subunit decreased to the baseline. Meanwhile, the expression of PML-RARalpha fusion protein obviously decreased after 24 hours, and kept low level at 48 hours. It is concluded that the expression of 26S proteasome beta(1)-subunit increases after exposure to As(2)O(3). Increment of 26S proteasome beta(1)-subunit may be associated with the degradation of PML-RARalpha fusion protein and plays roles in the differentiation and apoptosis of NB4 cells.
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Banco de datos:
MEDLINE
Asunto principal:
Óxidos
/
Arsenicales
/
Leucemia Promielocítica Aguda
/
Proteínas de Fusión Oncogénica
/
Apoptosis
/
Complejo de la Endopetidasa Proteasomal
Límite:
Humans
Idioma:
Zh
Revista:
Zhongguo Shi Yan Xue Ye Xue Za Zhi
Asunto de la revista:
HEMATOLOGIA
Año:
2009
Tipo del documento:
Article
País de afiliación:
China