Systematic artifacts in metagenomes from complex microbial communities.
ISME J
; 3(11): 1314-7, 2009 Nov.
Article
en En
| MEDLINE
| ID: mdl-19587772
ABSTRACT
Metagenomics is providing an unprecedented view of the taxonomic diversity, metabolic potential and ecological role of microbial communities in biomes as diverse as the mammalian gastrointestinal tract, the marine water column and soils. However, we have found a systematic error in metagenomes generated by 454-based pyrosequencing that leads to an overestimation of gene and taxon abundance; between 11% and 35% of sequences in a typical metagenome are artificial replicates. Here we document the error in several published and original datasets and offer a web-based solution (http//microbiomes.msu.edu/replicates) for identifying and removing these artifacts.
Texto completo:
1
Banco de datos:
MEDLINE
Asunto principal:
Microbiología del Suelo
/
Bases de Datos Genéticas
/
Metagenoma
/
Metagenómica
Tipo de estudio:
Prognostic_studies
Idioma:
En
Revista:
ISME J
Asunto de la revista:
MICROBIOLOGIA
/
SAUDE AMBIENTAL
Año:
2009
Tipo del documento:
Article
País de afiliación:
Estados Unidos