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Direct measurement of energy fluxes from mitochondria into cytoplasm in permeabilized cardiac cells in situ: some evidence for Mitochondrial Interactosome.
Timohhina, Natalia; Guzun, Rita; Tepp, Kersti; Monge, Claire; Varikmaa, Minna; Vija, Heiki; Sikk, Peeter; Kaambre, Tuuli; Sackett, Dan; Saks, Valdur.
Afiliación
  • Timohhina N; Laboratory of Bioenergetics, National Institute of Chemical Physics and Biophysics, Tallinn, Estonia.
J Bioenerg Biomembr ; 41(3): 259-75, 2009 Jun.
Article en En | MEDLINE | ID: mdl-19597977
The aim of this study was to measure energy fluxes from mitochondria in isolated permeabilized cardiomyocytes. Respiration of permeabilized cardiomyocytes and mitochondrial membrane potential were measured in presence of MgATP, pyruvate kinase - phosphoenolpyruvate and creatine. ATP and phosphocreatine concentrations in medium surrounding cardiomyocytes were determined. While ATP concentration did not change in time, mitochondria effectively produced phosphocreatine (PCr) with PCr/O(2) ratio equal to 5.68 +/- 0.14. Addition of heterodimeric tubulin to isolated mitochondria was found to increase apparent Km for exogenous ADP from 11 +/- 2 microM to 330 +/- 47 microM, but creatine again decreased it to 23 +/- 6 microM. These results show directly that under physiological conditions the major energy carrier from mitochondria into cytoplasm is PCr, produced by mitochondrial creatine kinase (MtCK), which functional coupling to adenine nucleotide translocase is enhanced by selective limitation of permeability of mitochondrial outer membrane within supercomplex ATP Synthasome-MtCK-VDAC-tubulin, Mitochondrial Interactosome.
Asunto(s)

Texto completo: 1 Banco de datos: MEDLINE Asunto principal: Metabolismo Energético / Potencial de la Membrana Mitocondrial / Mitocondrias Cardíacas Límite: Animals Idioma: En Revista: J Bioenerg Biomembr Año: 2009 Tipo del documento: Article País de afiliación: Estonia

Texto completo: 1 Banco de datos: MEDLINE Asunto principal: Metabolismo Energético / Potencial de la Membrana Mitocondrial / Mitocondrias Cardíacas Límite: Animals Idioma: En Revista: J Bioenerg Biomembr Año: 2009 Tipo del documento: Article País de afiliación: Estonia