Ultrahigh sensitivity made simple: nanoplasmonic label-free biosensing with an extremely low limit-of-detection for bacterial and cancer diagnostics.
Nanotechnology
; 20(43): 434015, 2009 Oct 28.
Article
en En
| MEDLINE
| ID: mdl-19801769
ABSTRACT
We present a simple and robust scheme for biosensing with an ultralow limit-of-detection down to several pg cm(-2) (or several tens of attomoles cm(-2)) based on optical label-free biodetection with localized surface plasmon resonances. The scheme utilizes cost-effective optical components and comprises a white light source, a properly functionalized sensor surface enclosed in a simple fluidics chip, and a spectral analyzer. The sensor surface is produced by a bottom-up nanofabrication technique with hole mask colloidal lithography. Despite its simplicity, the method is able to reliably detect protein-protein binding events at low picomolar and femtomolar concentrations, which is exemplified by the label-free detection of the extracellular adherence protein (EAP) found on the outer surface of the bacterium Staphylococcus aureus and of prostate-specific antigen (PSA), which is believed to be a prostate cancer marker. These experiments pave the way towards an ultra-sensitive yet compact biodetection platform for point-of-care diagnostics applications.
Texto completo:
1
Banco de datos:
MEDLINE
Asunto principal:
Staphylococcus aureus
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Proteínas Bacterianas
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Proteínas de Unión al ARN
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Antígeno Prostático Específico
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Resonancia por Plasmón de Superficie
Tipo de estudio:
Clinical_trials
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Diagnostic_studies
/
Evaluation_studies
Límite:
Humans
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Male
Idioma:
En
Revista:
Nanotechnology
Año:
2009
Tipo del documento:
Article
País de afiliación:
Suecia