The mechanisms of citrate on regulating the distribution of carbon flux in the biosynthesis of uridine 5'-monophosphate by Saccharomyces cerevisiae.
Appl Microbiol Biotechnol
; 86(1): 75-81, 2010 Mar.
Article
en En
| MEDLINE
| ID: mdl-19826805
ABSTRACT
A whole cell biocatalytic process for uridine 5'-monophosphate (UMP) production from orotic acid by Saccharomyces cerevisiae was developed. The concentration of UMP was increased by 23% when 1 g l(-1) sodium citrate was fed into the broth. Effects of citrate addition on UMP production were investigated. Glucose-6-phosphate pool was elevated by onefold, while FBP and pyruvate were decreased by 42% and 40%, respectively. Organic acid pools such as acetate and succinate were averagely decreased by 30% and 49%. The results demonstrated that manipulation of citrate levels could be used as a novel tool to regulate the metabolic fluxes distribution among glycolysis, pentose phosphate pathway, and TCA cycle.
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1
Banco de datos:
MEDLINE
Asunto principal:
Saccharomyces cerevisiae
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Uridina Monofosfato
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Biotecnología
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Regulación Enzimológica de la Expresión Génica
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Ácido Cítrico
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Glucosa
Idioma:
En
Revista:
Appl Microbiol Biotechnol
Año:
2010
Tipo del documento:
Article