[Effects of water stress on protein expression and physiological properties of different genotype wheat (Triticum aestivum L.) sprouts].

ABSTRACT

With drought-resistant wheat (Triticum aestivum) cultivar Changwu 134 and drought-sensitive cultivar Zhengyin 1 as test materials, and by using -1.2 MPa PEG 6000 to treat their seeds, this paper studied the protein expression and physiological properties of the sprouts under different soil moisture conditions. SDS-PAGE analysis showed that water stress induced the production of two new proteins with molecular weights of 39.5 kDa and 23.0 kDa in Changwu 134 but not in Zhengyin 1 sprouts. Under normal water supply, the expression of the protein with molecular weight of 48.5 kDa in the sprouts of both Changwu 134 and Zhengyin 1 increased with sprout growth. This protein was preliminarily named as water-sensitive protein, due to its water-sensitivity and of newly discovered protein. The determinations of physiological properties showed that under water stress, the sprouts of drought-resistant Changwu 134 had higher root/shoot ratio and higher relative water content, but lower relative membrane permeability and lower malondlaldehyde content than those of drought-sensitive Zhengyin 1.