Iothalamate quantification by tandem mass spectrometry to measure glomerular filtration rate.
Clin Chem
; 56(4): 568-74, 2010 Apr.
Article
en En
| MEDLINE
| ID: mdl-20167698
ABSTRACT
BACKGROUND:
Glomerular filtration rate (GFR) can be determined by measuring renal clearance of the radiocontrast agent iothalamate. Current analytic methods for quantifying iothalamate concentrations in plasma and urine using liquid chromatography or capillary electrophoresis have limitations such as long analysis times and susceptibility to interferences. We developed a liquid chromatography-tandem mass spectrometry (LC-MS/MS) method to overcome these limitations.METHODS:
Urine and plasma samples were deproteinized using acetonitrile and centrifugation. The supernatant was diluted in water and analyzed by LC-MS/MS using a watermethanol gradient. We monitored 4 multiple reaction monitoring transitions m/z 614.8-487.0, 614.8-456.0, 614.8-361.1, and 614.8-177.1. We compared the results to those obtained via our standard capillary electrophoresis (CE-UV) on samples from 53 patients undergoing clinical GFR testing.RESULTS:
Mean recovery was 90%-110% in both urine and plasma matrices. Imprecision wasCONCLUSIONS:
Iothalamate can be quantified by LC-MS/MS for GFR measurement. This method circumvents potential problems with interfering substances that occasionally confound accurate GFR determinations.
Texto completo:
1
Banco de datos:
MEDLINE
Asunto principal:
Tasa de Filtración Glomerular
/
Ácido Yotalámico
Tipo de estudio:
Diagnostic_studies
Límite:
Humans
Idioma:
En
Revista:
Clin Chem
Asunto de la revista:
QUIMICA CLINICA
Año:
2010
Tipo del documento:
Article
País de afiliación:
Estados Unidos