Novel approach for endothelializing vascular devices: understanding and exploiting elastin-endothelial interactions.
Ann Biomed Eng
; 39(1): 337-46, 2011 Jan.
Article
en En
| MEDLINE
| ID: mdl-20737290
ABSTRACT
Elastin is an essential component of arteries which provides structural integrity and instructs smooth muscle cells to adopt a quiescent state. Despite interaction of endothelial cells with elastin in the internal elastic lamina, the potential for exploiting this interaction therapeutically has not been explored in detail. In this study, we show that tropoelastin (a precursor of elastin) stimulates endothelial cell migration and adhesion more than smooth muscle cells. The biological activity of tropoelastin on endothelial cells is contained in the VGVAPG domain and in the carboxy-terminal 17-amino acids. We show that the effects of the carboxy-terminal 17 amino acids, but not those of VGVAPG, are mediated by integrin α(V)ß(3). We demonstrate that tropoelastin covalently linked to stainless steel disks promotes adhesion of endothelial progenitor cells and endothelial cells to the metal surfaces. The adherent cells on the tropoelastin-coated metal surfaces form monolayers that can withstand and respond to arterial shear stress. Because of the unique effects of tropoelastin on endothelial and smooth muscle cells, coating intravascular devices with tropoelastin may stimulate their endothelialization, inhibit smooth muscle hyperplasia, and improve device performance.
Texto completo:
1
Banco de datos:
MEDLINE
Asunto principal:
Movimiento Celular
/
Tropoelastina
/
Células Endoteliales
Límite:
Humans
Idioma:
En
Revista:
Ann Biomed Eng
Año:
2011
Tipo del documento:
Article
País de afiliación:
Estados Unidos