Lysosomal membrane permeabilization causes oxidative stress and ferritin induction in macrophages.
FEBS Lett
; 585(4): 623-9, 2011 Feb 18.
Article
en En
| MEDLINE
| ID: mdl-21219901
ABSTRACT
Moderate lysosomal membrane permeabilization (LMP) is an important inducer of apoptosis. Macrophages are professional scavengers and are rich in hydrolytic enzymes and iron. In the present study, we found that LMP by lysosomotropic detergent MSDH resulted in early up-regulation of lysosomal cathepsins, oxidative stress and ferritin up-regulation, and cell death. Lysosomotropic base NH(4)Cl reduced the ferritin induction and oxidative stress in apoptotic cells induced by MSDH. Cysteine cathepsin inhibitors significantly protected cell death and oxidative stress, but had less effect on ferritin induction. We conclude that oxidative stress induced by lysosomal rupture causes ferritin induction with concomitant mitochondrial damage, which are the potential target for prevention of cellular oxidative stress and cell death induced by typical lysosomotropic substances in different disorders.
Texto completo:
1
Banco de datos:
MEDLINE
Asunto principal:
Regulación hacia Arriba
/
Estrés Oxidativo
/
Ferritinas
/
Membranas Intracelulares
/
Lisosomas
/
Macrófagos
Tipo de estudio:
Etiology_studies
Límite:
Animals
/
Humans
Idioma:
En
Revista:
FEBS Lett
Año:
2011
Tipo del documento:
Article
País de afiliación:
Suecia