piRNA-associated germline nuage formation and spermatogenesis require MitoPLD profusogenic mitochondrial-surface lipid signaling.
Dev Cell
; 20(3): 376-87, 2011 Mar 15.
Article
en En
| MEDLINE
| ID: mdl-21397848
ABSTRACT
The mammalian Phospholipase D MitoPLD facilitates mitochondrial fusion by generating the signaling lipid phosphatidic acid (PA). The Drosophila MitoPLD homolog Zucchini (Zuc), a proposed cytoplasmic nuclease, is required for piRNA generation, a critical event in germline development. We show that Zuc localizes to mitochondria and has MitoPLD-like activity. Conversely, MitoPLD(-/-) mice exhibit the meiotic arrest, DNA damage, and male sterility characteristic of mice lacking piRNAs. The primary function of MitoPLD seems to be the generation of mitochondrial-surface PA. This PA in turn recruits the phosphatase Lipin 1, which converts PA to diacylglycerol and promotes mitochondrial fission, suggesting a mechanism for mitochondrial morphology homeostasis. MitoPLD and Lipin 1 have opposing effects on mitochondria length and on intermitochondrial cement (nuage), a structure found between aggregated mitochondria that is implicated in piRNA generation. We propose that mitochondrial-surface PA generated by MitoPLD/Zuc recruits or activates nuage components critical for piRNA production.
Texto completo:
1
Banco de datos:
MEDLINE
Asunto principal:
Fosfolipasa D
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Espermatogénesis
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Transducción de Señal
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Proteínas de Drosophila
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Proteínas Mitocondriales
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ARN Interferente Pequeño
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Endorribonucleasas
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Células Germinativas
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Mitocondrias
Tipo de estudio:
Risk_factors_studies
Idioma:
En
Revista:
Dev Cell
Asunto de la revista:
EMBRIOLOGIA
Año:
2011
Tipo del documento:
Article
País de afiliación:
Estados Unidos