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A competitive ELISA for the detection of group-specific antibody to equine encephalosis virus.
Crafford, J E; Guthrie, A J; Van Vuuren, M; Mertens, P P C; Burroughs, J N; Howell, P G; Batten, C A; Hamblin, C.
Afiliación
  • Crafford JE; Department of Veterinary Tropical Diseases, University of Pretoria, Onderstepoort 0110, South Africa.
J Virol Methods ; 174(1-2): 60-4, 2011 Jun.
Article en En | MEDLINE | ID: mdl-21458496
ABSTRACT
A polyclonal antibody-based, group-specific, competitive ELISA (C-ELISA) for the detection of antibodies to equine encephalosis virus (EEV) was developed. The assay measures the competition between a specific guinea pig antiserum and a test serum, for a pre-titrated EEV antigen. The C-ELISA detected antibodies to the seven known EEV serotypes. Reference antisera raised against other arboviruses did not cross react with EEV antigen. Negative sera from horses in the United Kingdom were used to establish the baseline for a negative population. Negative and positive populations of South African horses, selected on the basis of virus neutralisation were assayed subsequently. Optimal test parameters, where sensitivity≅specificity≅100%, were calculated by two-graph receiver operator characteristic (TG-ROC) analysis to be at a cut-off value of 29.5% inhibition. Results show the EEV C-ELISA described to be sensitive, specific and reliable. Used in conjunction with ELISAs available for African horse sickness virus (AHSV), differential serological diagnosis between EEV and AHSV can be achieved.
Asunto(s)

Texto completo: 1 Banco de datos: MEDLINE Asunto principal: Virología / Orbivirus / Infecciones por Reoviridae / Técnicas de Laboratorio Clínico / Enfermedades de los Caballos / Anticuerpos Antivirales Tipo de estudio: Diagnostic_studies / Evaluation_studies Límite: Animals País/Región como asunto: Africa / Europa Idioma: En Revista: J Virol Methods Año: 2011 Tipo del documento: Article País de afiliación: Sudáfrica

Texto completo: 1 Banco de datos: MEDLINE Asunto principal: Virología / Orbivirus / Infecciones por Reoviridae / Técnicas de Laboratorio Clínico / Enfermedades de los Caballos / Anticuerpos Antivirales Tipo de estudio: Diagnostic_studies / Evaluation_studies Límite: Animals País/Región como asunto: Africa / Europa Idioma: En Revista: J Virol Methods Año: 2011 Tipo del documento: Article País de afiliación: Sudáfrica