Comparison of five antimicrobial susceptibility tests in detecting high level aminoglycoside and vancomycin resistances in hospital acquired Enterococcus isolates.
Clin Lab
; 57(3-4): 157-62, 2011.
Article
en En
| MEDLINE
| ID: mdl-21500722
ABSTRACT
BACKGROUND:
Multiple antibiotic resistance is increasing in the genus Enterococcus. The aim of this research is to compare five different antibiotic susceptibility test methods used in enterococcal isolates.METHODS:
Disc diffusion, agar dilution, Etest, and API Enteroc 5 tests were compared with the standard antimicrobial susceptibility test (SAST) (broth microdilution) in 100 Enterococcus strains isolated from various clinical specimens.RESULTS:
The resistance rates of the isolates to antibiotics were as follows 51% resistance to tetracycline, 38% to erythromycin, 28% to penicillin and ampicillin, 23% to high level gentamicin, 16% to high level streptomycin, 14% to chloramphenicol, 9% to ciprofloxacin, and 1% to nitrofurantoine, as determined by SAST. Except a moderate-susceptible vancomycin strain, all strains were found to be susceptible to vancomycin and teicoplanin. None of the strains examined had beta-lactamase enzyme activity. Of significance, one major error in detecting high level gentamicin and two serious errors in detecting high level streptomycin resistances were detected when API was compared with the SAST method. Major errors were also detected in penicillin, erythromycin, tetracycline, ciprofloxacin, and nitrofurantoin with the API method.CONCLUSIONS:
Disc diffusion, agar dilution, SAST, and Etest methods were equally reliable for the detection of all antimicrobials studied and the disc diffusion method is considered easy to perform and inexpensive method. The API method is considered unreliable in detecting high level aminoglycoside resistance.
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Banco de datos:
MEDLINE
Asunto principal:
Farmacorresistencia Microbiana
/
Pruebas de Sensibilidad Microbiana
/
Infección Hospitalaria
/
Enterococcus
/
Resistencia a la Vancomicina
/
Farmacorresistencia Bacteriana Múltiple
Límite:
Humans
Idioma:
En
Revista:
Clin Lab
Asunto de la revista:
TECNICAS E PROCEDIMENTOS DE LABORATORIO
Año:
2011
Tipo del documento:
Article
País de afiliación:
Turquía