Expression of recombinant Araraquara Hantavirus nucleoprotein in insect cells and its use as an antigen for immunodetection compared to the same antigen expressed in Escherichia coli.
Virol J
; 8: 218, 2011 May 11.
Article
en En
| MEDLINE
| ID: mdl-21569341
ABSTRACT
BACKGROUND:
Antigens for Hantavirus serological tests have been produced using DNA recombinant technology for more than twenty years. Several different strategies have been used for that purpose. All of them avoid the risks and difficulties involved in multiplying Hantavirus in the laboratory. In Brazil, the Araraquara virus is one of the main causes of Hantavirus Cardio-Pulmonary Syndrome (HCPS).METHODS:
In this investigation, we report the expression of the N protein of the Araraquara Hantavirus in a Baculovirus Expression System, the use of this protein in IgM and IgG ELISA and comparison with the same antigen generated in E. coli.RESULTS:
The protein obtained, and purified in a nickel column, was effectively recognized by antibodies from confirmed HCPS patients. Comparison of the baculovirus generated antigen with the N protein produced in E. coli showed that both were equally effective in terms of sensitivity and specificity.CONCLUSIONS:
Our results therefore indicate that either of these proteins can be used in serological tests in Brazil.
Texto completo:
1
Banco de datos:
MEDLINE
Asunto principal:
Virología
/
Baculoviridae
/
Orthohantavirus
/
Infecciones por Hantavirus
/
Técnicas de Laboratorio Clínico
/
Antígenos Virales
/
Nucleoproteínas
Tipo de estudio:
Diagnostic_studies
/
Evaluation_studies
Límite:
Animals
/
Humans
País/Región como asunto:
America do sul
/
Brasil
Idioma:
En
Revista:
Virol J
Asunto de la revista:
VIROLOGIA
Año:
2011
Tipo del documento:
Article
País de afiliación:
Brasil