ER-α36, a novel variant of estrogen receptor α, is involved in EGFR-related carcinogenesis in endometrial cancer.
Am J Obstet Gynecol
; 205(3): 227.e1-6, 2011 Sep.
Article
en En
| MEDLINE
| ID: mdl-21684519
ABSTRACT
OBJECTIVE:
To explore the role of estrogen receptor-α36 (ER-α36) in epidermal growth factor receptor (EGFR)-related carcinogenesis in endometrial cancer. STUDYDESIGN:
The expression of ER-α36, EGFR, and phospho-extracellular signal-regulated kinase was analyzed using immunohistochemistry in endometrial cancer samples. The cellular localization of ER-α36 and EGFR was determined using immunofluorescence in the endometrial cancer Hec1A cells. The level of phospho-extracellular signal-regulated kinase of Hec1A cells was determined using Western blotting after treatment with epidermal growth factor.RESULTS:
Positive rate of ER-α36 was increased in high-stage (P = .03) and high-grade (P = .224) endometrial cancer; expression of ER-α36 and EGFR exhibited a significant positive correlation (r = 0.334, P = .025) and they showed substantial colocalization on the plasma membrane of glandular cells; phospho-extracellular signal-regulated kinase positive rate in ER-α36 positive group and EGFR positive group was higher than that of ER-α36 negative group (P = .014) and EGFR negative group (P = .016); finally, ER-α36 mediated epidermal growth factor-stimulated extracellular signal-regulated kinase activation in Hec1A cells.CONCLUSION:
ER-α36 mediates EGFR-related extracellular signal-regulated kinase activation in endometrial cancer.
Texto completo:
1
Banco de datos:
MEDLINE
Asunto principal:
Neoplasias Endometriales
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Receptor alfa de Estrógeno
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Receptores ErbB
Límite:
Female
/
Humans
Idioma:
En
Revista:
Am J Obstet Gynecol
Año:
2011
Tipo del documento:
Article
País de afiliación:
China