Notch lineages and activity in intestinal stem cells determined by a new set of knock-in mice.
PLoS One
; 6(10): e25785, 2011.
Article
en En
| MEDLINE
| ID: mdl-21991352
ABSTRACT
The conserved role of Notch signaling in controlling intestinal cell fate specification and homeostasis has been extensively studied. Nevertheless, the precise identity of the cells in which Notch signaling is active and the role of different Notch receptor paralogues in the intestine remain ambiguous, due to the lack of reliable tools to investigate Notch expression and function in vivo. We generated a new series of transgenic mice that allowed us, by lineage analysis, to formally prove that Notch1 and Notch2 are specifically expressed in crypt stem cells. In addition, a novel Notch reporter mouse, Hes1-EmGFP(SAT), demonstrated exclusive Notch activity in crypt stem cells and absorptive progenitors. This roster of knock-in and reporter mice represents a valuable resource to functionally explore the Notch pathway in vivo in virtually all tissues.
Texto completo:
1
Banco de datos:
MEDLINE
Asunto principal:
Células Madre
/
Linaje de la Célula
/
Receptores Notch
/
Técnicas de Sustitución del Gen
/
Intestinos
Límite:
Animals
Idioma:
En
Revista:
PLoS One
Asunto de la revista:
CIENCIA
/
MEDICINA
Año:
2011
Tipo del documento:
Article
País de afiliación:
Francia