Effects of different activation regimens on pronuclear formation and developmental competence of in vitro-matured porcine oocytes after intracytoplasmic sperm injection.
Reprod Domest Anim
; 47(4): 609-14, 2012 Aug.
Article
en En
| MEDLINE
| ID: mdl-22039996
ABSTRACT
To improve the activation protocol for in vitro-maturated porcine oocytes after intracytoplasmic sperm injection (ICSI), we examined the combined effect of U0126, a specific inhibitor of mitogen-activated protein kinase kinases, and an electrical pulse on pronuclear formation and developmental competence. Two approaches were tested (i) 6-h treatment of ICSI oocytes with U0126 applied at different intervals (0, 2, 3 or 4 h) after the electrical pulse and (ii) treatment of ICSI oocytes with U0126 applied 4 h after the electrical pulse over an additional 4, 6 or 8 h. Another protein kinase inhibitor, 6-dimethylaminopurine, was used as a chemical activator in control experiments. The highest rates of diploid embryo formation, normal fertilization and blastocyst formation were observed after 6 h of exposure to U0126 starting 4 h after the electrical pulse. Therefore, U0126 can be used as an activating agent for porcine oocytes fertilized by ICSI.
Texto completo:
1
Banco de datos:
MEDLINE
Asunto principal:
Oocitos
/
Porcinos
/
Inyecciones de Esperma Intracitoplasmáticas
/
Técnicas de Maduración In Vitro de los Oocitos
Tipo de estudio:
Guideline
Límite:
Animals
Idioma:
En
Revista:
Reprod Domest Anim
Asunto de la revista:
MEDICINA REPRODUTIVA
/
MEDICINA VETERINARIA
Año:
2012
Tipo del documento:
Article
País de afiliación:
China