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Restrictions in cell cycle progression of adult vestibular supporting cells in response to ectopic cyclin D1 expression.
Loponen, Heidi; Ylikoski, Jukka; Albrecht, Jeffrey H; Pirvola, Ulla.
Afiliación
  • Loponen H; Institute of Biotechnology, University of Helsinki, Helsinki, Finland.
PLoS One ; 6(11): e27360, 2011.
Article en En | MEDLINE | ID: mdl-22073316
Sensory hair cells and supporting cells of the mammalian inner ear are quiescent cells, which do not regenerate. In contrast, non-mammalian supporting cells have the ability to re-enter the cell cycle and produce replacement hair cells. Earlier studies have demonstrated cyclin D1 expression in the developing mouse supporting cells and its downregulation along maturation. In explant cultures of the mouse utricle, we have here focused on the cell cycle control mechanisms and proliferative potential of adult supporting cells. These cells were forced into the cell cycle through adenoviral-mediated cyclin D1 overexpression. Ectopic cyclin D1 triggered robust cell cycle re-entry of supporting cells, accompanied by changes in p27(Kip1) and p21(Cip1) expressions. Main part of cell cycle reactivated supporting cells were DNA damaged and arrested at the G2/M boundary. Only small numbers of mitotic supporting cells and rare cells with signs of two successive replications were found. Ectopic cyclin D1-triggered cell cycle reactivation did not lead to hyperplasia of the sensory epithelium. In addition, a part of ectopic cyclin D1 was sequestered in the cytoplasm, reflecting its ineffective nuclear import. Combined, our data reveal intrinsic barriers that limit proliferative capacity of utricular supporting cells.
Asunto(s)

Texto completo: 1 Banco de datos: MEDLINE Asunto principal: Ciclo Celular / Vestíbulo del Laberinto / Ciclina D1 Límite: Adult / Humans Idioma: En Revista: PLoS One Asunto de la revista: CIENCIA / MEDICINA Año: 2011 Tipo del documento: Article País de afiliación: Finlandia

Texto completo: 1 Banco de datos: MEDLINE Asunto principal: Ciclo Celular / Vestíbulo del Laberinto / Ciclina D1 Límite: Adult / Humans Idioma: En Revista: PLoS One Asunto de la revista: CIENCIA / MEDICINA Año: 2011 Tipo del documento: Article País de afiliación: Finlandia