Expression of membrane-associated proteins within single emulsion cell facsimiles.
Analyst
; 137(13): 2939-43, 2012 Jul 07.
Article
en En
| MEDLINE
| ID: mdl-22441306
MreB is a structural membrane-associated protein which is one of the key components of the bacterial cytoskeleton. Although it plays an important role in shape maintenance of rod-like bacteria, the understanding of its mechanism of action is still not fully understood. This study shows how segmented flow and microdroplet technology can be used as a new tool for biological in vitro investigation of this protein. In this paper, we demonstrate cell-free expression in a single emulsion system to express red fluorescence protein (RFP) and MreB linked RFP (MreB-RFP). We follow the aggregation and localisation of the fusion protein MreB-RFP in this artificial cell-like environment. The expression of MreB-RFP in single emulsion droplets leads to the formation of micrometer-scale protein patches distributed at the water/oil interface.
Texto completo:
1
Banco de datos:
MEDLINE
Asunto principal:
Emulsiones
/
Proteínas de la Membrana
Tipo de estudio:
Risk_factors_studies
Idioma:
En
Revista:
Analyst
Año:
2012
Tipo del documento:
Article