An optimized two-finger archive for ZFN-mediated gene targeting.
Nat Methods
; 9(6): 588-90, 2012 Jun.
Article
en En
| MEDLINE
| ID: mdl-22543349
ABSTRACT
The widespread use of zinc-finger nucleases (ZFNs) for genome engineering is hampered by the fact that only a subset of sequences can be efficiently recognized using published finger archives. We describe a set of validated two-finger modules that complement existing finger archives and expand the range of ZFN-accessible sequences threefold. Using this archive, we introduced lesions at 9 of 11 target sites in the zebrafish genome.
Texto completo:
1
Banco de datos:
MEDLINE
Asunto principal:
Dedos de Zinc
/
Marcación de Gen
Límite:
Animals
Idioma:
En
Revista:
Nat Methods
Asunto de la revista:
TECNICAS E PROCEDIMENTOS DE LABORATORIO
Año:
2012
Tipo del documento:
Article
País de afiliación:
Estados Unidos