Investigation of two-photon excited fluorescence increment via crosslinked bovine serum albumin.
Opt Express
; 20(13): 13669-76, 2012 Jun 18.
Article
en En
| MEDLINE
| ID: mdl-22714432
ABSTRACT
The two-photon excited fluorescence (TPEF) increments of two dyes via bovine serum albumin (BSA) microstructures fabricated by the two-photon crosslinking technique were investigated. One is Rose Bengal (RB) with a high non-radiative decay rate, while the other is Eosin Y with a low non-radiative decay rate. Experimental results demonstrate that the quantum yield and lifetime of RB are both augmented via crosslinked BSA microstructures. Compared with theoretical analysis, this result indicates that the non-radiative decay rate of RB is decreased; hence, the quenched effect induced by BSA solution is suppressed. However, the fluorescence lifetime of Eosin Y is acutely abated despite the augmented quantum yield for the two-photon crosslinking processing from BSA solution. This result deduces that the radiative decay rate increased. Furthermore, the increased TPEF intensity and lifetime of RB correlated with the concentration of fabricated crosslinked BSA microstructures through pulse selection of the employed femtosecond laser is demonstrated and capable of developing a zone-plate-like BSA microstructure.
Texto completo:
1
Banco de datos:
MEDLINE
Asunto principal:
Rosa Bengala
/
Albúmina Sérica Bovina
/
Eosina Amarillenta-(YS)
/
Microscopía de Fluorescencia por Excitación Multifotónica
Idioma:
En
Revista:
Opt Express
Asunto de la revista:
OFTALMOLOGIA
Año:
2012
Tipo del documento:
Article
País de afiliación:
Taiwán