Xenopus transgenics: methods using transposons.
Methods Mol Biol
; 917: 231-43, 2012.
Article
en En
| MEDLINE
| ID: mdl-22956092
ABSTRACT
The generation of transgenic animals is an essential tool for many genetic strategies. DNA "cut-and-paste" transposon systems can be used to efficiently modify the Xenopus genome. The DNA transposon substrate, harbored on a circularized plasmid, is co-injected into fertilized Xenopus embryos at the one-cell stage together with mRNA encoding the cognate transposase enzyme. The cellular machinery rapidly translates the exogenous mRNA to produce active transposase enzyme that catalyzes excision of the transposon substrate from the plasmid and stable integration into the genomic DNA.
Texto completo:
1
Banco de datos:
MEDLINE
Asunto principal:
Xenopus
/
Elementos Transponibles de ADN
/
Mutagénesis Insercional
Límite:
Animals
Idioma:
En
Revista:
Methods Mol Biol
Asunto de la revista:
BIOLOGIA MOLECULAR
Año:
2012
Tipo del documento:
Article
País de afiliación:
Estados Unidos