IL-17A in the tumor microenvironment of the human colorectal adenoma-carcinoma sequence.
Scand J Gastroenterol
; 47(11): 1304-12, 2012 Nov.
Article
en En
| MEDLINE
| ID: mdl-22989213
ABSTRACT
OBJECTIVES:
Interleukin (IL)-17A is an important pro-inflammatory cytokine and involved in the colorectal carcinogenesis. In this study, the authors evaluated the dynamic change of IL-17A expression in the tumor microenvironment throughout the colorectal adenoma-carcinoma sequence. MATERIALS &METHODS:
Using quantitative real-time PCR (polymerase chain reaction) and semi-quantitative immunohistochemistry, the authors examined the expression level of IL-17A in 50 of human colorectal adenoma tissues, 50 of colorectal cancer (CRC) tissues and 15 controls. The relationship between IL-17A expression and clinicopathological parameters throughout the sequence was also evaluated.RESULTS:
The results revealed a step-up increased IL-17A mRNA level throughout the colorectal adenoma-carcinoma sequence, which began to increase in the adenomas and became even higher in the CRCs; notably, the increase of IL-17A mRNA level in the adenomatous tissues was associated with the severity of dysplasia. Immunohistochemical analysis confirmed the real-time PCR results and revealed gradually increasing IL-17A cells in both the stroma and adenomatous/cancerous epithelium. In addition, the quantitative real-time PCR result has also revealed an increased expression of TH17-stimulating factors throughout the sequence.CONCLUSIONS:
IL-17A and TH17 are highly activated throughout the colorectal adenoma-carcinoma sequence.
Texto completo:
1
Banco de datos:
MEDLINE
Asunto principal:
Carcinoma
/
Neoplasias Colorrectales
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Adenoma
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Interleucina-17
Tipo de estudio:
Observational_studies
/
Risk_factors_studies
Límite:
Adult
/
Aged
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Aged80
/
Female
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Humans
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Male
/
Middle aged
Idioma:
En
Revista:
Scand J Gastroenterol
Año:
2012
Tipo del documento:
Article
País de afiliación:
China