CaWRKY40, a WRKY protein of pepper, plays an important role in the regulation of tolerance to heat stress and resistance to Ralstonia solanacearum infection.
Plant Cell Environ
; 36(4): 757-74, 2013 Apr.
Article
en En
| MEDLINE
| ID: mdl-22994555
ABSTRACT
WRKY proteins form a large family of plant transcription factors implicated in the modulation of numerous biological processes, such as growth, development and responses to various environmental stresses. However, the roles of the majority WRKY family members, especially in non-model plants, remain poorly understood. We identified CaWRKY40 from pepper. Transient expression in onion epidermal cells showed that CaWRKY40 can be targeted to nuclei and activates expression of a W-box-containing reporter gene. CaWRKY40 transcripts are induced in pepper by Ralstonia solanacearum and heat shock. To assess roles of CaWRKY40 in plant stress responses we performed gain- and loss-of-function experiments. Overexpression of CaWRKY40 enhanced resistance to R. solanacearum and tolerance to heat shock in tobacco. In contrast, silencing of CaWRKY40 enhanced susceptibility to R. solanacearum and impaired thermotolerance in pepper. Consistent with its role in multiple stress responses, we found CaWRKY40 transcripts to be induced by signalling mechanisms mediated by the stress hormones salicylic acid (SA), jasmonic acid (JA) and ethylene (ET). Overexpression of CaWRKY40 in tobacco modified the expression of hypersensitive response (HR)-associated and pathogenesis-related genes. Collectively, our results suggest that CaWRKY40 orthologs are regulated by SA, JA and ET signalling and coordinate responses to R. solanacearum attacks and heat stress in pepper and tobacco.
Texto completo:
1
Banco de datos:
MEDLINE
Asunto principal:
Enfermedades de las Plantas
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Reguladores del Crecimiento de las Plantas
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Factores de Transcripción
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Capsicum
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Regulación de la Expresión Génica de las Plantas
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Ralstonia solanacearum
Tipo de estudio:
Prognostic_studies
Idioma:
En
Revista:
Plant Cell Environ
Asunto de la revista:
BOTANICA
Año:
2013
Tipo del documento:
Article
País de afiliación:
China