TAML activator-based amperometric analytical devices as alternatives to peroxidase biosensors.
Anal Chem
; 84(21): 9096-100, 2012 Nov 06.
Article
en En
| MEDLINE
| ID: mdl-23005918
ABSTRACT
The ferric TAML catalysts [Fe{C(6)H(2)-1,2-( NCOCMe(2)NCO)(2)CMe(2)}(OH(2))](-) (1) with counterions Na(+) (a) and PPh(4)(+) (b) function similar to horseradish peroxidase in the mediated electron transfer relays, which constitute a basis for amperometric biosensors. The mediators are mono- and bis-cyclometalated Ru and Os compounds of the type of [M(Câ¼N)(x)(Nâ¼N)(3-x)](m+) with x = 1 and 2 (Nâ¼N = 2,2'-bipyridine, (-)Câ¼N = 2-phenylpyridinato). Cyclic voltammograms of the Ru and Os compounds are not affected by 1a though cathodic currents increase drastically in the presence of hydrogen peroxide. The reduction potentials of [M(Câ¼N)(x)(Nâ¼N)(3-x)](m+) complexes vary with both the nature of metal (Ru or Os) and the number of cyclometalated ligands x (1 or 2) and therefore the potential of working electrode can be set in the range of from -0.1 to +0.6 V versus the normal hydrogen electrode (NHE). A prototype of a biosensor for H(2)O(2) is described, in which the 1b catalyst and [Os(Câ¼N)(2)(Nâ¼N)](+) mediator were coimmobilized on the surface of the glassy carbon electrode using a polymeric coating.
Texto completo:
1
Banco de datos:
MEDLINE
Asunto principal:
Compuestos Férricos
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Técnicas Biosensibles
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Electroquímica
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Peroxidasa de Rábano Silvestre
Idioma:
En
Revista:
Anal Chem
Año:
2012
Tipo del documento:
Article
País de afiliación:
Estados Unidos