Mitochondrial DNA as effective molecular markers for the genetic variation and phylogeny of the family Osteoglossidae.
Gene
; 511(2): 320-5, 2012 Dec 15.
Article
en En
| MEDLINE
| ID: mdl-23036711
The present study examined the genetic variation of the family Osteoglossidae from different geographical locations based on the mitochondrial NADH dehydrogenase subunit 2 (ND2) and ATPase subunit 6 (ATPase6) genes; we then re-constructed the phylogenetic relationships using the two sequences in combination. The results showed that the partial sequences of mitochondrial ND2 and ATPase6 of the family Osteoglossidae were 813 bp and 669 bp, respectively. A total of 42 species-specific nucleotide positions of the family Osteoglossidae were found to be useful for molecular identification. The sequence variation showed greater differences (8.3%~28.1% for the combined sequences, 8.3%~26.7% for the ND2 gene, and 9.3%~28.7% for the ATPase6 gene) among the different species of Osteoglossidae, and there was a significant association between the genetic difference and geographical location. Phylogenetic analyses using neighbor-joining, Bayesian inference, and maximum parsimony (MP) methods based on the combined sequences of the two genes were able to distinguish the different species and were in agreement with the existing taxonomy based on morphological characters and in association with the geographical distribution among seven species of the family Osteoglossidae.
Texto completo:
1
Banco de datos:
MEDLINE
Asunto principal:
Filogenia
/
Variación Genética
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ADN Mitocondrial
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Peces
Límite:
Animals
Idioma:
En
Revista:
Gene
Año:
2012
Tipo del documento:
Article
País de afiliación:
China