Using intravital microscopy to observe bevacizumab-mediated anti-angiogenesis in human head and neck squamous cell carcinoma xenografts.
Acta Otolaryngol
; 132(12): 1324-33, 2012 Dec.
Article
en En
| MEDLINE
| ID: mdl-23039202
CONCLUSION: The study showed the value of using intravital microscopy (IVM) analysis for the study of neoangiogenesis. It demonstrated that the model and the analytical methodology could be used to evaluate in detail the effects of treatment strategies for solid tumours. OBJECTIVES: Neoangiogenesis is a key component of tumour progression, invasion and metastasis. In clinical trials monoclonal antibodies specific for vascular endothelial growth factor - VEGF (bevacizumab) - have been shown to significantly affect tumour progression when given in combination with standard chemotherapy, and also to improve the overall survival of patients. For squamous cell carcinoma of the head and neck (HNSCC), we still await definitive evidence of the effect of such treatment. The present study was designed to investigate the anti-angiogenesis effect of beviacizumab in green fluorescent protein (GFP)-labelled HNSCC xenografts using IVM technology. METHODS: We performed IVM and used image analysis for quantification of angiogenesis and of effects of bevacizumab on cell viability, combined with histochemical and immunohistochemical analysis to standardize the digital analysis of changes in tumour vascularization and cell viability. RESULTS: We found significant effects of bevacizumab on angiogenesis and cancer cell survival in HNSCC. Repeated injections of bevacizumab were found to provide the greatest effects.
Texto completo:
1
Banco de datos:
MEDLINE
Asunto principal:
Neoplasias de Oído, Nariz y Garganta
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Carcinoma de Células Escamosas
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Técnica de Ventana Cutánea
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Inhibidores de la Angiogénesis
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Dermoscopía
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Modelos Animales de Enfermedad
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Anticuerpos Monoclonales Humanizados
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Trasplante de Neoplasias
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Neovascularización Patológica
Tipo de estudio:
Prognostic_studies
Límite:
Animals
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Humans
Idioma:
En
Revista:
Acta Otolaryngol
Año:
2012
Tipo del documento:
Article
País de afiliación:
Japón