NDRG2 and PRA1 interact and synergistically inhibit T-cell factor/ß-catenin signaling.
FEBS Lett
; 586(22): 3962-8, 2012 Nov 16.
Article
en En
| MEDLINE
| ID: mdl-23068607
ABSTRACT
NDRG2 is a member of the N-myc downstream regulated gene (NDRG) family, implicated in cell growth and differentiation. Investigation of NDRG2 molecular interactions by yeast two-hybrid screening identified prenylated Rab acceptor-1 (PRA1), involved in vesicle trafficking and protein transport, as binding partner. Binding of NDRG2 (and NDRG1-4) with PRA1 in vitro was confirmed by GST pull-down assay and immunoprecipitation, and colocalization was verified by confocal microscopy in HCT116 cells. Intracellular coexpression showed that NDRG2 and PRA1 synergistically downregulate T-cell factor (TCF) promoter activity and GSK3ß phosphorylation. Results suggest that NDRG2 and PRA1 might act synergistically to prevent signaling of TCF/ß-catenin.
Texto completo:
1
Banco de datos:
MEDLINE
Asunto principal:
Transducción de Señal
/
Proteínas de Unión al GTP
/
Proteínas Supresoras de Tumor
/
Proteínas de Transporte Vesicular
/
Beta Catenina
/
Factores de Transcripción TCF
Tipo de estudio:
Prognostic_studies
Límite:
Humans
Idioma:
En
Revista:
FEBS Lett
Año:
2012
Tipo del documento:
Article