[Relationship between genetic variation of furin and insulin resistance in Chinese Kazakh population].

OBJECTIVE: To investigate the relationship between genetic variation of Furin and insulin resistance in Chinese Kazakh population. METHODS: Based on a cross-sectional epidemiological study in a Chinese Kazakh population, a case-control study was conducted. All the sequence variants located promoter and exon regions of Furin were identified by directly sequencing of PCR product in 50 (25 males) individuals with insulin resistance, which were randomly chosen from the study population. The representative polymorphism was detected by TaqMan PCR in 861 subjects (366 males, 254 in case group and 607 in control group). The relationship between genetic variation of Furin and insulin resistance in this cohort was analyzed. RESULTS: Twelve genetic variations in Furin were identified by sequencing 50 individuals with insulin resistance and 4 common SNPs (rs6226, rs6227, rs2071410, and rs4932178) were selected as representatives for genotyping in this Chinese Kazakh population. The rs6226, rs6227, rs2071410, and rs4932178 polymorphisms were successfully genotyped. The distribution of the genotypes of rs6226, rs6227, rs2071410, and rs4932178 polymorphism was similar between case and controls (all P > 0.05). The homeostasis model assessment for insulin resistance (HOMA-IR) levels was also similar among individuals with different genotypes (all P > 0.05). CONCLUSION: The genetic variation of Furin is not associated with insulin resistance in this cohort of Chinese Kazakh population.