Investigation of isotope dilution mass spectrometric (ID-MS) method to determine niacin in infant formula, breakfast cereals and multivitamins.
Food Chem
; 138(2-3): 1109-15, 2013 Jun 01.
Article
en En
| MEDLINE
| ID: mdl-23411221
ABSTRACT
An isotope dilution LC/mass spectrometric (ID-LC/MS) method was developed as a candidate reference method for the accurate determination of niacin in infant formula, breakfast cereals and multivitamin. After spiking nicotinamide-d(4) as an internal standard, infant formula and breakfast cereal samples were hydrolysed under alkaline condition. Samples were then analysed in SRM mode to detect nicotinic acid and nicotinic acid-d(4) at m/z 124â80 and 127â84, respectively. In the case of multivitamin sample that contains mainly free nicotinamide, LC/MS monitored nicotinamide and nicotinamide-d(4) at their SRM channels of m/z 123â80 and m/z 127â84, respectively, after simple extraction. The repeatability and reproducibility were tested for the validation of the developed ID/LC-MS method. Additionally, the developed analytical method was applied to determine total niacin contents in homogenised infant formula, homogenised multivitamin, and commercially available products including different types of infant formula, breakfast cereals, and multivitamin tablets.
Texto completo:
1
Banco de datos:
MEDLINE
Asunto principal:
Espectrometría de Masas
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Contaminación de Alimentos
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Grano Comestible
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Suplementos Dietéticos
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Fórmulas Infantiles
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Niacina
Tipo de estudio:
Evaluation_studies
Idioma:
En
Revista:
Food Chem
Año:
2013
Tipo del documento:
Article