The application of the Cre-loxP system for generating multiple knock-out and knock-in targeted loci.
Methods Mol Biol
; 983: 249-67, 2013.
Article
en En
| MEDLINE
| ID: mdl-23494311
ABSTRACT
Dictyostelium discoideum is an exceptionally powerful eukaryotic model to study many aspects of growth, development, and fundamental cellular processes. Its small-sized, haploid genome allows highly efficient targeted homologous recombination for gene disruption and knock-in epitope tagging. We previously described a robust system for the generation of multiple gene mutations in Dictyostelium by recycling the Blasticidin S selectable marker after transient expression of the Cre recombinase. We have now further optimized the system for higher efficiency and, additionally, coupled it to both, knock-out and knock-in gene targeting, allowing the characterization of multiple and cooperative gene functions in a single cell line.
Texto completo:
1
Banco de datos:
MEDLINE
Asunto principal:
Integrasas
/
Dictyostelium
/
Técnicas de Sustitución del Gen
/
Técnicas de Inactivación de Genes
Idioma:
En
Revista:
Methods Mol Biol
Asunto de la revista:
BIOLOGIA MOLECULAR
Año:
2013
Tipo del documento:
Article
País de afiliación:
Alemania