Protocol: ex vivo culture of mouse embryonic mammary buds.
J Mammary Gland Biol Neoplasia
; 18(2): 239-45, 2013 Jun.
Article
en En
| MEDLINE
| ID: mdl-23674216
ABSTRACT
The explant culture techniques of embryonic tissues allow continuous monitoring of organ growth and morphogenesis ex vivo. The effect of growth factors and other soluble molecules can be examined by applying them to the culture medium. Relatively few studies have reported application of tissue culture techniques to analysis of embryonic mammary glands. Here we describe a protocol for murine mammary rudiments that permits ex vivo development up to branching stage.
Texto completo:
1
Banco de datos:
MEDLINE
Asunto principal:
Técnicas de Cultivo de Órganos
/
Glándulas Mamarias Humanas
Límite:
Animals
/
Humans
Idioma:
En
Revista:
J Mammary Gland Biol Neoplasia
Asunto de la revista:
NEOPLASIAS
Año:
2013
Tipo del documento:
Article
País de afiliación:
Finlandia