Persistently betanodavirus-infected barramundi (Lates calcarifer) exhibit resistances to red sea bream iridovirus infection.
Dev Comp Immunol
; 41(4): 666-74, 2013 Dec.
Article
en En
| MEDLINE
| ID: mdl-23831937
ABSTRACT
Nervous necrosis virus (NNV) and red sea bream iridovirus (RSIV) are two important pathogens that have caused acute, highly contagious, and widespread diseases among wild and cultured fish, especially at larval and juvenile stages. We discovered that the pathogenicity of NNV to the 80 days post-hatch (dph) barramundi is lower than that to the 14 dph barramundi. Following NNV challenge, no mortality occurred in the 80 dph barramundi, but NNV RNA2 and barramundi Mx (BMx) gene expression was detected in the brain and liver. The 80 dph barramundi pre-challenged with NNV became more resistant to the following RSIV challenge (mortality 62%) compared to the NNV-free barramundi challenged with RSIV (mortality 100%). A similar phenomenon was revealed in the cell culture system that RSIV proliferated less progeny in the barramundi brain (BB) cell line which exhibit persistent NNV infection than in NNV-free cured BB (cBB) cell line. The potential factors involved in the resistance of the persistently NNV-infected barramundi and BB cells to the secondary RSIV infection were examined in this study. We prove that barramundi anti-NNV polyclonal antibodies do not cross-neutralize RSIV, and NNV infection does not interfere with RSIV replication. However, the interferon (IFN) response and BMx gene expression in cBB cells suppresses the RSIV proliferation. Our study suggests that the NNV-induced IFN response and BMx expression are responsible for the resistance of barramundi to RSIV infection.
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Texto completo:
1
Banco de datos:
MEDLINE
Asunto principal:
Infecciones por Virus ARN
/
Perciformes
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Iridovirus
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Dorada
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Nodaviridae
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Enfermedades de los Peces
Límite:
Animals
Idioma:
En
Revista:
Dev Comp Immunol
Año:
2013
Tipo del documento:
Article
País de afiliación:
Taiwán