Use of thiamine pyrophosphate to prevent infertility developing in rats undergoing unilateral ovariectomy and with ischemia reperfusion induced in the contralateral ovary.
Eur J Obstet Gynecol Reprod Biol
; 170(2): 521-5, 2013 Oct.
Article
en En
| MEDLINE
| ID: mdl-23916585
ABSTRACT
OBJECTIVE:
To investigate whether thiamine pyrophosphate can prevent infertility developing in rats undergoing unilateral ovariectomy and with ischemia reperfusion induced in the contralateral ovary. Biochemical examinations of the ovaries were also performed. STUDYDESIGN:
Rats were divided into two main groups of three subgroups each. An ischemia reperfusion model was established in the first main group, while surgical unilateral ovariectomy was performed in the second. Thiamine pyrophosphate and melatonin were administered to the subgroups. No additional procedure was performed in the control groups. The rats were then left in laboratory environments and their fertility levels were determined. Malondialdehyde, total glutathione and DNA damage products were measured in those rats from which ovarian tissue was collected.RESULTS:
The results showed that thiamine pyrophosphate prevented ischemia/reperfusion injury-related infertility, but melatonin did not provide adequate prevention. However, reproduction in healthy animals receiving melatonin began earlier compared to those receiving thiamine pyrophosphate. Melatonin suppressed oxidative stress caused by ischemia/reperfusion in ovarian tissue significantly better than did thiamine pyrophosphate.CONCLUSIONS:
We think that different mechanisms, in addition to antioxidant activity, are involved in the prevention of reperfusion-associated infertility after ischemia.Palabras clave
Texto completo:
1
Banco de datos:
MEDLINE
Asunto principal:
Enfermedades del Ovario
/
Tiamina Pirofosfato
/
Complejo Vitamínico B
/
Daño por Reperfusión
/
Infertilidad Femenina
Tipo de estudio:
Etiology_studies
Límite:
Animals
Idioma:
En
Revista:
Eur J Obstet Gynecol Reprod Biol
Año:
2013
Tipo del documento:
Article
País de afiliación:
Turquía