Loop-mediated isothermal amplification method for a differential identification of human Taenia tapeworms.
Methods Mol Biol
; 1039: 109-20, 2013.
Article
en En
| MEDLINE
| ID: mdl-24026690
ABSTRACT
Loop-mediated isothermal amplification (LAMP), which employs a Bst DNA polymerase with strand-displacement activity and four primers (two inner primers and two outer primers) recognizing six distinct regions on the target DNA, is a highly sensitive, specific, simple, and rapid nucleotide amplification method. Moreover, because the Bst DNA polymerase resists much DNA polymerase inhibitors present in biological specimens, the LAMP method is suitable for the detection of infectious agents from clinical material such as fecal samples. Here, we describe the LAMP method which can differentially detect and identify human Taenia tapeworms, Taenia solium, T. saginata, and T. asiatica, using DNA specimens prepared from parasite tissue and human fecal sample.
Texto completo:
1
Banco de datos:
MEDLINE
Asunto principal:
Teniasis
/
Técnicas de Amplificación de Ácido Nucleico
/
Taenia saginata
Tipo de estudio:
Diagnostic_studies
/
Prognostic_studies
Límite:
Animals
/
Humans
Idioma:
En
Revista:
Methods Mol Biol
Asunto de la revista:
BIOLOGIA MOLECULAR
Año:
2013
Tipo del documento:
Article
País de afiliación:
Japón