Barcoding T cell calcium response diversity with methods for automated and accurate analysis of cell signals (MAAACS).
PLoS Comput Biol
; 9(9): e1003245, 2013.
Article
en En
| MEDLINE
| ID: mdl-24086124
ABSTRACT
We introduce a series of experimental procedures enabling sensitive calcium monitoring in T cell populations by confocal video-microscopy. Tracking and post-acquisition analysis was performed using Methods for Automated and Accurate Analysis of Cell Signals (MAAACS), a fully customized program that associates a high throughput tracking algorithm, an intuitive reconnection routine and a statistical platform to provide, at a glance, the calcium barcode of a population of individual T-cells. Combined with a sensitive calcium probe, this method allowed us to unravel the heterogeneity in shape and intensity of the calcium response in T cell populations and especially in naive T cells, which display intracellular calcium oscillations upon stimulation by antigen presenting cells.
Texto completo:
1
Banco de datos:
MEDLINE
Asunto principal:
Programas Informáticos
/
Linfocitos T
/
Transducción de Señal
/
Calcio
Límite:
Animals
/
Humans
Idioma:
En
Revista:
PLoS Comput Biol
Asunto de la revista:
BIOLOGIA
/
INFORMATICA MEDICA
Año:
2013
Tipo del documento:
Article
País de afiliación:
Francia