Human liver sinusoidal endothelial cells but not hepatocytes contain factor VIII.
J Thromb Haemost
; 12(1): 36-42, 2014 Jan.
Article
en En
| MEDLINE
| ID: mdl-24118899
ABSTRACT
BACKGROUND:
Although the liver is the major site of coagulation factor VIII (FVIII) synthesis, the type of cells producing FVIII within the liver is still unclear.OBJECTIVES:
To measure FVIII in extracts of primary liver sinusoidal endothelial cells (LSECs) and hepatocytes, thereby preventing potential bias resulting from the modifications of the cell phenotype that can take place during in vitro culture.METHODS:
LSECs were purified by flow cytometry cell sorting on the basis of their coexpression of Tie2 and CD32b. The purity of the cells was controlled by RNA sequencing. FVIII activity (FVIIIC) in extracts of purified cells was measured with a sensitive FVIII chromogenic assay, in which the specificity of the reaction is controlled by neutralization of FVIII activity with specific inhibitor antibodies.RESULTS:
The FVIIIC concentration in purified LSECs ranged from 0.3 to 2.8 nU per cell. In contrast, FVIIIC was undetectable in hepatocytes. The intracellular FVIIIC concentrations are therefore at least 10-100-fold higher in LSECs than in hepatocytes.CONCLUSIONS:
Our data demonstrate that LSECs, but not hepatocytes, contain measurable amounts of FVIIIC, and suggest that the former are the main cells producing FVIII in the human liver.Palabras clave
Texto completo:
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Banco de datos:
MEDLINE
Asunto principal:
Factor VIII
/
Hepatocitos
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Células Endoteliales
/
Hígado
Límite:
Humans
Idioma:
En
Revista:
J Thromb Haemost
Asunto de la revista:
HEMATOLOGIA
Año:
2014
Tipo del documento:
Article
País de afiliación:
Irán