EatA, an immunogenic protective antigen of enterotoxigenic Escherichia coli, degrades intestinal mucin.
Infect Immun
; 82(2): 500-8, 2014 Feb.
Article
en En
| MEDLINE
| ID: mdl-24478066
ABSTRACT
Enterotoxigenic Escherichia coli (ETEC) is a major cause of morbidity and mortality due to infectious diarrhea in developing countries for which there is presently no effective vaccine. A central challenge in ETEC vaccinology has been the identification of conserved surface antigens to formulate a broadly protective vaccine. Here, we demonstrate that EatA, an immunogenic secreted serine protease of ETEC, contributes to virulence by degrading MUC2, the major protein present in the small intestinal mucous layer, and that removal of this barrier in vitro accelerates toxin access to the enterocyte surface. In addition, we demonstrate that vaccination with the recombinant secreted passenger domain of EatA (rEatAp) elicits high titers of antibody and is protective against intestinal infection with ETEC. These findings may have significant implications for development of both subunit and live-attenuated vaccines against ETEC and other enteric pathogens, including Shigella flexneri, that express similar proteins.
Texto completo:
1
Banco de datos:
MEDLINE
Asunto principal:
Proteínas Portadoras
/
Proteínas de Escherichia coli
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Factores de Virulencia
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Escherichia coli Enterotoxigénica
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Mucina 2
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Antígenos Bacterianos
Tipo de estudio:
Prognostic_studies
Límite:
Animals
/
Humans
Idioma:
En
Revista:
Infect Immun
Año:
2014
Tipo del documento:
Article
País de afiliación:
Estados Unidos