Reverse transcription and subsequent DNA amplification of rubella virus RNA.
J Virol Methods
; 25(1): 21-9, 1989 Jul.
Article
en En
| MEDLINE
| ID: mdl-2476457
ABSTRACT
A method is described whereby rubella virus RNA was reverse transcribed and the resulting cDNA enzymatically amplified using Taq polymerase. The reactions were carried out in a single reaction vessel, with only minor modifications to the buffer conditions between the reverse transcription and the subsequent amplification step. Using an oligonucleotide probe to the E1 glycoprotein region and limited restriction endonuclease mapping, the resulting amplified products were shown to be specific for rubella virus. This method was also successfully applied to crude cell lysates, without the need for RNA purification. The possible applications of the polymerase chain reaction as applied to RNA sequences are discussed.
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Banco de datos:
MEDLINE
Asunto principal:
Virus de la Rubéola
/
Transcripción Genética
/
ARN Viral
/
Amplificación de Genes
/
ADN Polimerasa Dirigida por ARN
Límite:
Animals
/
Humans
Idioma:
En
Revista:
J Virol Methods
Año:
1989
Tipo del documento:
Article
País de afiliación:
Sudáfrica