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Preparation and identification of transfer factor specific to Staphylococcus aureus in vitro.
Zhou, Jianwei; Kong, Cui; Wang, Xiukui; Shao, Jun; Feng, Lin; Zhang, Zhaocai.
Afiliación
  • Zhou J; Clinical Laboratory, The Affiliated Hospital of Jining Medical College, Jining, Shandong, People's Republic of China.
Biotechnol Appl Biochem ; 62(1): 112-20, 2015.
Article en En | MEDLINE | ID: mdl-24825752
The objective of the study was to explore the methods for preparing transfer factor specific to Staphylococcus aureus (SA-STF) in vitro. Under the optimum conditions, the spleen cells of rabbits were immunized with SA in vitro to prepare SA-STF, and the immune activities were identified with the phagocytosis and sterilization, skin delayed-type hypersensitivity, and immune protection tests. The concentration of polypeptide was 2.26 ± 0.27 mg/mL, and ribose was 0.684 ± 0.094 mg/mL. The phagocytosis and sterilization rates of the STF group were 70.9 ± 12.4% and 62.1 ± 12.2%, respectively, and compared with the non-specific transfer factor (NTF) group, there were no significant differences (P = 0.074 and 0.069, respectively). The skin was inflamed and marked nodules formed at the injection site in the mice of the STF group rather than the NTF and control groups. The survival rate of the STF-1 group was significantly higher than the survival rates of the STF-2 (P = 0.024) and NTF groups (P = 0.016). SA-STF was prepared and characterized successfully in vitro, and it probably is a biological candidate for therapy or adjuvant therapy for diseases caused by Staphylococcus aureus.
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Texto completo: 1 Banco de datos: MEDLINE Asunto principal: Staphylococcus aureus / Factor de Transferencia Tipo de estudio: Diagnostic_studies / Prognostic_studies Límite: Animals Idioma: En Revista: Biotechnol Appl Biochem Asunto de la revista: BIOQUIMICA / BIOTECNOLOGIA Año: 2015 Tipo del documento: Article

Texto completo: 1 Banco de datos: MEDLINE Asunto principal: Staphylococcus aureus / Factor de Transferencia Tipo de estudio: Diagnostic_studies / Prognostic_studies Límite: Animals Idioma: En Revista: Biotechnol Appl Biochem Asunto de la revista: BIOQUIMICA / BIOTECNOLOGIA Año: 2015 Tipo del documento: Article