Rapid detection of predation of Escherichia coli O157:H7 and sorting of bacterivorous Tetrahymena by flow cytometry.
Front Cell Infect Microbiol
; 4: 57, 2014.
Article
en En
| MEDLINE
| ID: mdl-24847471
ABSTRACT
Protozoa are known to harbor bacterial pathogens, alter their survival in the environment and make them hypervirulent. Rapid non-culture based detection methods are required to determine the environmental survival and transport of enteric pathogens from point sources such as dairies and feedlots to food crops grown in proximity. Grazing studies were performed on a soil isolate of Tetrahymena fed green fluorescent protein (GFP) expressing Escherichia coli O157H7 to determine the suitability of the use of such fluorescent prey bacteria to locate and sort bacterivorous protozoa by flow cytometry. In order to overcome autofluorescence of the target organism and to clearly discern Tetrahymena with ingested prey vs. those without, a ratio of prey to host of at least 1001 was determined to be preferable. Under these conditions, we successfully sorted the two populations using short 5-45 min exposures of the prey and verified the internalization of E. coli O157H7 cells in protozoa by confocal microscopy. This technique can be easily adopted for environmental monitoring of rates of enteric pathogen destruction vs. protection in protozoa.
Palabras clave
Texto completo:
1
Banco de datos:
MEDLINE
Asunto principal:
Tetrahymena
/
Escherichia coli O157
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Microbiología Ambiental
/
Citometría de Flujo
Tipo de estudio:
Diagnostic_studies
Idioma:
En
Revista:
Front Cell Infect Microbiol
Año:
2014
Tipo del documento:
Article
País de afiliación:
Estados Unidos