Temporal focusing-based multiphoton excitation microscopy via digital micromirror device.
Opt Lett
; 39(11): 3134-7, 2014 Jun 01.
Article
en En
| MEDLINE
| ID: mdl-24875995
ABSTRACT
This Letter presents an enhanced temporal focusing-based multiphoton excitation (MPE) microscope in which the conventional diffraction grating is replaced by a digital micromirror device (DMD). Experimental results from imaging a thin fluorescence film show that the 4.0 µm axial resolution of the microscope is comparable with that of a setup incorporating a 600 lines/mm grating; hence, the optical sectioning ability of the proposed setup is demonstrated. Similar to a grating, the DMD diffracts illuminating light frequencies for temporal focusing; additionally, it generates arbitrary patterns. Since the DMD is placed on the image-conjugate plane of the objective lens' focal plane, the MPE pattern can be projected on the focal plane precisely.
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Banco de datos:
MEDLINE
Asunto principal:
Microscopía de Fluorescencia por Excitación Multifotónica
Idioma:
En
Revista:
Opt Lett
Año:
2014
Tipo del documento:
Article