Molecular characterization of Thai Salmonella enterica serotype typhimurium and serotype 4,5,12:i:- reveals distinct genetic deletion patterns.
Foodborne Pathog Dis
; 11(8): 589-92, 2014 Aug.
Article
en En
| MEDLINE
| ID: mdl-24906076
ABSTRACT
In order to better understand the relationship between Salmonella serotypes Typhimurium and its monophasic variant 4,5,12i- found in Thailand, a total of 138 isolates from various sources were characterized using different molecular subtyping methods (i.e., pulsed-field gel electrophoresis [PFGE] and polymerase chain reaction [PCR]) and antibiotic resistance (AbR) patterns. PFGE revealed 52 distinct band patterns among these isolates, 3 of which were shared between the two serotypes. PCR characterization of genomic deletion patterns reveals that Thai S. 4,5,12i- isolates contain a distinct deletion pattern in the fljAB region, which can be used as a specific genetic marker for primary identification of S. 4,5,12i- sources. AbR study shows that, among 50 representative serotype-confirmed strains, 48.28% (14/29) of Salmonella Typhimurium and 90.48% (19/21) of 4,5,12i- isolates are multidrug-resistant Salmonella as they are resistant to at least 3 antimicrobial categories. The AmpST pattern for resistance to ampicillin, streptomycin, and tetracycline was found in high proportions of Salmonella Typhimurium (10 of 29) and S. 4,5,12i- (15 of 21) isolates.
Texto completo:
1
Banco de datos:
MEDLINE
Asunto principal:
Salmonella typhimurium
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Eliminación de Gen
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Farmacorresistencia Bacteriana Múltiple
País/Región como asunto:
Asia
Idioma:
En
Revista:
Foodborne Pathog Dis
Asunto de la revista:
CIENCIAS DA NUTRICAO
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MICROBIOLOGIA
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PARASITOLOGIA
Año:
2014
Tipo del documento:
Article
País de afiliación:
Tailandia