Mapping of polar fox renal cortex proteins using two-dimensional gel electrophoresis and mass spectrometry--a preliminary study.
Pol J Vet Sci
; 17(2): 231-7, 2014.
Article
en En
| MEDLINE
| ID: mdl-24988848
ABSTRACT
The aim of the present study was to establish protein map of polar fox (Alopex lagopus) renal cortex. Kidney cortex proteins of isoelectric point ranging from 3 to 10 were analysed using two-dimensional electrophoresis and MALDI-TOF mass spectrometry. Sixteen protein spots corresponding to thirteen different gene products were identified. These proteins were divided into following groups lipid and fatty acid metabolism, amino acid metabolism, energetic pathways, regulatory proteins, transport proteins and structural proteins. This is the first attempt to create reproducible 2-D map, of renal cortex proteins characteristic for polar foxes, used as animal model for carnivores. It is worth emphasizing that the results of this study may broaden currently available protein databases.
Buscar en Google
Banco de datos:
MEDLINE
Asunto principal:
Espectrometría de Masas
/
Electroforesis en Gel Bidimensional
/
Proteínas
/
Zorros
/
Corteza Renal
Tipo de estudio:
Prognostic_studies
Límite:
Animals
Idioma:
En
Revista:
Pol J Vet Sci
Asunto de la revista:
MEDICINA VETERINARIA
Año:
2014
Tipo del documento:
Article