P22 mediated recombination of frt-sites.
Virology
; 462-463: 340-2, 2014 Aug.
Article
en En
| MEDLINE
| ID: mdl-25019493
ABSTRACT
Flp mediated site specific recombination of frt-sites is frequently used in genetic engineering to excise, insert or invert DNA-cassettes in the chromosome. While constructs flanked by frt-sites are generally considered to be stable in the absence of the Flp enzyme, we observed that P22 chromosomes exceeding wild-type length tend to lose frt-flanked insertions via Flp independent recombination of frt-sites during phage propagation. This spontaneous recombination should be considered when engineering the chromosome of P22 and perhaps of other phages as well.
Palabras clave
Texto completo:
1
Banco de datos:
MEDLINE
Asunto principal:
Recombinación Genética
/
ADN Viral
/
Bacteriófago P22
Idioma:
En
Revista:
Virology
Año:
2014
Tipo del documento:
Article
País de afiliación:
Bélgica