PDE7A1 hydrolyzes cCMP.
FEBS Lett
; 588(18): 3469-74, 2014 Sep 17.
Article
en En
| MEDLINE
| ID: mdl-25128584
The degradation and biological role of the cyclic pyrimidine nucleotide cCMP is largely elusive. We investigated nucleoside 3',5'-cyclic monophosphate (cNMP) specificity of six different recombinant phosphodiesterases (PDEs) by using a highly-sensitive HPLC-MS/MS detection method. PDE7A1 was the only enzyme that hydrolyzed significant amounts of cCMP. Enzyme kinetic studies using purified GST-tagged truncated PDE7A1 revealed a cCMP KM value of 135 ± 19 µM. The Vmax for cCMP hydrolysis reached 745 ± 27 nmol/(minmg), which is about 6-fold higher than the corresponding velocity for adenosine 3',5'-cyclic monophosphate (cAMP) degradation. In summary, PDE7A is a high-speed and low-affinity PDE for cCMP.
Palabras clave
Texto completo:
1
Banco de datos:
MEDLINE
Asunto principal:
CMP Cíclico
/
Fosfodiesterasas de Nucleótidos Cíclicos Tipo 7
Límite:
Animals
/
Humans
Idioma:
En
Revista:
FEBS Lett
Año:
2014
Tipo del documento:
Article
País de afiliación:
Alemania