From genes to protein mechanics on a chip.
Nat Methods
; 11(11): 1127-1130, 2014 Nov.
Article
en En
| MEDLINE
| ID: mdl-25194847
ABSTRACT
Single-molecule force spectroscopy enables mechanical testing of individual proteins, but low experimental throughput limits the ability to screen constructs in parallel. We describe a microfluidic platform for on-chip expression, covalent surface attachment and measurement of single-molecule protein mechanical properties. A dockerin tag on each protein molecule allowed us to perform thousands of pulling cycles using a single cohesin-modified cantilever. The ability to synthesize and mechanically probe protein libraries enables high-throughput mechanical phenotyping.
Texto completo:
1
Banco de datos:
MEDLINE
Asunto principal:
Análisis de Secuencia por Matrices de Oligonucleótidos
/
Análisis por Matrices de Proteínas
/
Técnicas Analíticas Microfluídicas
Idioma:
En
Revista:
Nat Methods
Asunto de la revista:
TECNICAS E PROCEDIMENTOS DE LABORATORIO
Año:
2014
Tipo del documento:
Article
País de afiliación:
Alemania