let-7 miRNAs can act through notch to regulate human gliogenesis.
Stem Cell Reports
; 3(5): 758-73, 2014 Nov 11.
Article
en En
| MEDLINE
| ID: mdl-25316189
ABSTRACT
It is clear that neural differentiation from human pluripotent stem cells generates cells that are developmentally immature. Here, we show that the let-7 plays a functional role in the developmental decision making of human neural progenitors, controlling whether these cells make neurons or glia. Through gain- and loss-of-function studies on both tissue and pluripotent derived cells, our data show that let-7 specifically regulates decision making in this context by regulation of a key chromatin-associated protein, HMGA2. Furthermore, we provide evidence that the let-7/HMGA2 circuit acts on HES5, a NOTCH effector and well-established node that regulates fate decisions in the nervous system. These data link the let-7 circuit to NOTCH signaling and suggest that this interaction serves to regulate human developmental progression.
Texto completo:
1
Banco de datos:
MEDLINE
Asunto principal:
Neuroglía
/
Células Madre Pluripotentes
/
MicroARNs
/
Receptores Notch
Tipo de estudio:
Prognostic_studies
Límite:
Humans
Idioma:
En
Revista:
Stem Cell Reports
Año:
2014
Tipo del documento:
Article
País de afiliación:
Estados Unidos