[Effect of cerebral X-ray irradiation on learning and memory function in young SD rats].
Zhejiang Da Xue Xue Bao Yi Xue Ban
; 43(5): 535-40, 2014 09.
Article
en Zh
| MEDLINE
| ID: mdl-25372637
ABSTRACT
OBJECTIVE:
To investigate the effect of cerebral X-ray irradiation on learning and memory function in young rats.METHODS:
Fifty-four SD rats aged 35 d were randomly divided into 3 groups with 18 in each group rats in 3-d group and 7-d group received X-ray irradiation with a dose of 28.5 mGy/d for 3 d and 7 d, respectively; rats in control group received sham X-ray irradiation. Morris water maze (MWM) was tested when animals at age of 60 d; then the animals were sacrificed and brain samples were taken. The neurodegeneration was observed by Fluro-Jade B staining; the expression of N-methyl-aspartate (NMDA) receptors subunit 2B (NR2B) and postsynaptic density protein-95 (PSD-95) in the hippocampus were analyzed by immunofluorescence and Western blot methods, respectively, and ultrastructure of CA1 region was observed with electron microscopy.RESULTS:
No significant difference in 1-4 d escape latency as shown in MWM test was noted between 3d group and control group (P>0.05); while the escape latency in 7d group was significantly longer than that in control group (P<0.01). No significant differences in lingering in the quadrant and the frequency of passing through the original platform between 3-d group and control group (P>0.05), while those in 7-d group were significantly lower than those in control group (P<0.01). Compared to control group, the number of FJB positive cells in 7-d group was increased (P<0.01); the expressions of NR2B and PSD-95 in hippocampus CA1 region were also increased (P<0.05). The ultrastructure observation in 7-d group showed that the synapse structure of some neurons was impaired.CONCLUSION:
X-ray irradiation may affect learning and memory function of young rats, which is associated with overexpression of NR2B and PSD-95 in hippocampal regions.
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Banco de datos:
MEDLINE
Asunto principal:
Encéfalo
/
Aprendizaje
/
Memoria
Límite:
Animals
Idioma:
Zh
Revista:
Zhejiang Da Xue Xue Bao Yi Xue Ban
Asunto de la revista:
MEDICINA
Año:
2014
Tipo del documento:
Article