Combining solvent isotope effects with substrate isotope effects in mechanistic studies of alcohol and amine oxidation by enzymes.
Biochim Biophys Acta
; 1854(11): 1746-55, 2015 Nov.
Article
en En
| MEDLINE
| ID: mdl-25448013
ABSTRACT
Oxidation of alcohols and amines is catalyzed by multiple families of flavin- and pyridine nucleotide-dependent enzymes. Measurement of solvent isotope effects provides a unique mechanistic probe of the timing of the cleavage of the OH and NH bonds, necessary information for a complete description of the catalytic mechanism. The inherent ambiguities in interpretation of solvent isotope effects can be significantly decreased if isotope effects arising from isotopically labeled substrates are measured in combination with solvent isotope effects. The application of combined solvent and substrate (mainly deuterium) isotope effects to multiple enzymes is described here to illustrate the range of mechanistic insights that such an approach can provide. This article is part of a Special Issue entitled Enzyme Transition States from Theory and Experiment.
Palabras clave
Texto completo:
1
Banco de datos:
MEDLINE
Asunto principal:
Solventes
/
Alcoholes
/
Enzimas
/
Aminas
Idioma:
En
Revista:
Biochim Biophys Acta
Año:
2015
Tipo del documento:
Article